A folic acid labelled carbon quantum dot-protoporphryin IX conjugate for use in folate receptor targeted two-photon excited photodynamic therapy

Dean Nicholas, Colin Fowley, Anthony P. McHale, Sukanta Kamila, Jason Sheng, Jordan Atchison, John F. Callan

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

4 Citations (Scopus)

Abstract

Folic acid (FA) has been used as a molecular targeting strategy to improve the specificity of a CQD-protoporphyrin IX (CQD-PPIX) conjugate to folate receptor positive (FR+) HeLa cells for use in two-photon excited Photodynamic Therapy (TPE-PDT). FA was covalently attached to the CQD-PPIX conjugate to form a FA-CQD-PPIX conjugate. The uptake of the FA-CQD-PPIX conjugate in FR+ HeLa cells was shown to be 7 times greater than the CQD-PPIX conjugate, while both conjugates showed a similar uptake in FR negative (FR-) HT-47 cells. TPE-PDT experiments, using HeLa cells as a target, revealed a 30% improved cytotoxicity for cells treated with the FA-CQD-PPIX conjugate and TPE compared to controls treated with the CQD-PPIX conjugate and TPE. Collectively, these results suggest the presence of FA can facilitate targeting of CQD-sensitiser conjugates to FR+ cells resulting in an improved PDT effect.

Original languageEnglish
Title of host publicationColloidal Nanoparticles for Biomedical Applications X
EditorsWolfgang J. Parak, Marek Osinski, Xing-Jie Liang
PublisherSPIE
ISBN (Electronic)9781628414288
DOIs
Publication statusPublished - 2015
Externally publishedYes
EventColloidal Nanoparticles for Biomedical Applications X - San Francisco, United States
Duration: 7 Feb 20159 Feb 2015

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9338
ISSN (Print)1605-7422

Conference

ConferenceColloidal Nanoparticles for Biomedical Applications X
Country/TerritoryUnited States
CitySan Francisco
Period7/02/159/02/15

Keywords

  • Carbon Qunatum Dot
  • Folic Acid
  • PDT
  • Protoporphyrin IX

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