Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains

Reeta Davis; Rashmi Kataria; Federico Cerrone; Trevor Woods; Shane Kenny; Anthonia O'Donovan; Maciej Guzik; Hamid Shaikh; Gearoid Duane; Vijai Kumar Gupta; Maria G. Tuohy; Ramesh Babu Padamatti; Eoin Casey; Kevin E. O'Connor

doi:10.1016/j.biortech.2013.10.001

Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains

Reeta Davis, Rashmi Kataria, Federico Cerrone, Trevor Woods, Shane Kenny, Anthonia O'Donovan, Maciej Guzik, Hamid Shaikh, Gearoid Duane, Vijai Kumar Gupta, Maria G. Tuohy, Ramesh Babu Padamatti, Eoin Casey, Kevin E. O'Connor

Research output: Contribution to journal › Article › peer-review

129 Citations (Scopus)

Abstract

This study investigated the potential of grass biomass as a feedstock for mcl-PHA production. Pretreatments (2% NaOH at 120. °C or hot water at 120. °C) of perennial ryegrass were employed alone or in combination with sodium chlorite/acetic acid (SC/AA) delignification to evaluate the enzymatic digestibility and subsequent utilization of resultant sugars by Pseudomonas strains. NaOH pretreated sample had better digestibility than raw and hot water treated samples and this hydrolysate supported good growth of all tested strains with limited mcl-PHA (6-17% of cell dry mass (CDM)) accumulation. Digestibility of both untreated and pretreated samples was improved after SC/AA delignification and produced glucose (74-77%) rich hydrolysates. Tested strains accumulated 20-34% of CDM as PHA when these hydrolysates were used as sole carbon and energy source. CDM and PHA yields obtained for these strains when tested with laboratory grade sugars was similar to that achieved with grass derived sugars.

Original language	English
Pages (from-to)	202-209
Number of pages	8
Journal	Bioresource Technology
Volume	150
DOIs	https://doi.org/10.1016/j.biortech.2013.10.001
Publication status	Published - Dec 2013
Externally published	Yes

Keywords

Biomass
Enzyme hydrolyses
Mcl-Polyhydroxyalkanoates
Perennial ryegrass
Pre-treatment

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2013.10.001

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Davis, R., Kataria, R., Cerrone, F., Woods, T., Kenny, S., O'Donovan, A., Guzik, M., Shaikh, H., Duane, G., Gupta, V. K., Tuohy, M. G., Padamatti, R. B., Casey, E., & O'Connor, K. E. (2013). Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains. Bioresource Technology, 150, 202-209. https://doi.org/10.1016/j.biortech.2013.10.001

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title = "Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains",

abstract = "This study investigated the potential of grass biomass as a feedstock for mcl-PHA production. Pretreatments (2% NaOH at 120. °C or hot water at 120. °C) of perennial ryegrass were employed alone or in combination with sodium chlorite/acetic acid (SC/AA) delignification to evaluate the enzymatic digestibility and subsequent utilization of resultant sugars by Pseudomonas strains. NaOH pretreated sample had better digestibility than raw and hot water treated samples and this hydrolysate supported good growth of all tested strains with limited mcl-PHA (6-17% of cell dry mass (CDM)) accumulation. Digestibility of both untreated and pretreated samples was improved after SC/AA delignification and produced glucose (74-77%) rich hydrolysates. Tested strains accumulated 20-34% of CDM as PHA when these hydrolysates were used as sole carbon and energy source. CDM and PHA yields obtained for these strains when tested with laboratory grade sugars was similar to that achieved with grass derived sugars.",

keywords = "Biomass, Enzyme hydrolyses, Mcl-Polyhydroxyalkanoates, Perennial ryegrass, Pre-treatment",

author = "Reeta Davis and Rashmi Kataria and Federico Cerrone and Trevor Woods and Shane Kenny and Anthonia O'Donovan and Maciej Guzik and Hamid Shaikh and Gearoid Duane and Gupta, {Vijai Kumar} and Tuohy, {Maria G.} and Padamatti, {Ramesh Babu} and Eoin Casey and O'Connor, {Kevin E.}",

year = "2013",

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doi = "10.1016/j.biortech.2013.10.001",

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volume = "150",

pages = "202--209",

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Davis, R, Kataria, R, Cerrone, F, Woods, T, Kenny, S, O'Donovan, A, Guzik, M, Shaikh, H, Duane, G, Gupta, VK, Tuohy, MG, Padamatti, RB, Casey, E & O'Connor, KE 2013, 'Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains', Bioresource Technology, vol. 150, pp. 202-209. https://doi.org/10.1016/j.biortech.2013.10.001

TY - JOUR

T1 - Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains

AU - Davis, Reeta

AU - Kataria, Rashmi

AU - Cerrone, Federico

AU - Woods, Trevor

AU - Kenny, Shane

AU - O'Donovan, Anthonia

AU - Guzik, Maciej

AU - Shaikh, Hamid

AU - Duane, Gearoid

AU - Gupta, Vijai Kumar

AU - Tuohy, Maria G.

AU - Padamatti, Ramesh Babu

AU - Casey, Eoin

AU - O'Connor, Kevin E.

PY - 2013/12

Y1 - 2013/12

N2 - This study investigated the potential of grass biomass as a feedstock for mcl-PHA production. Pretreatments (2% NaOH at 120. °C or hot water at 120. °C) of perennial ryegrass were employed alone or in combination with sodium chlorite/acetic acid (SC/AA) delignification to evaluate the enzymatic digestibility and subsequent utilization of resultant sugars by Pseudomonas strains. NaOH pretreated sample had better digestibility than raw and hot water treated samples and this hydrolysate supported good growth of all tested strains with limited mcl-PHA (6-17% of cell dry mass (CDM)) accumulation. Digestibility of both untreated and pretreated samples was improved after SC/AA delignification and produced glucose (74-77%) rich hydrolysates. Tested strains accumulated 20-34% of CDM as PHA when these hydrolysates were used as sole carbon and energy source. CDM and PHA yields obtained for these strains when tested with laboratory grade sugars was similar to that achieved with grass derived sugars.

AB - This study investigated the potential of grass biomass as a feedstock for mcl-PHA production. Pretreatments (2% NaOH at 120. °C or hot water at 120. °C) of perennial ryegrass were employed alone or in combination with sodium chlorite/acetic acid (SC/AA) delignification to evaluate the enzymatic digestibility and subsequent utilization of resultant sugars by Pseudomonas strains. NaOH pretreated sample had better digestibility than raw and hot water treated samples and this hydrolysate supported good growth of all tested strains with limited mcl-PHA (6-17% of cell dry mass (CDM)) accumulation. Digestibility of both untreated and pretreated samples was improved after SC/AA delignification and produced glucose (74-77%) rich hydrolysates. Tested strains accumulated 20-34% of CDM as PHA when these hydrolysates were used as sole carbon and energy source. CDM and PHA yields obtained for these strains when tested with laboratory grade sugars was similar to that achieved with grass derived sugars.

KW - Biomass

KW - Enzyme hydrolyses

KW - Mcl-Polyhydroxyalkanoates

KW - Perennial ryegrass

KW - Pre-treatment

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U2 - 10.1016/j.biortech.2013.10.001

DO - 10.1016/j.biortech.2013.10.001

M3 - Article

C2 - 24177152

AN - SCOPUS:84886432275

SN - 0960-8524

VL - 150

SP - 202

EP - 209

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Conversion of grass biomass into fermentable sugars and its utilization for medium chain length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas strains

Abstract

Keywords

UN SDGs

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