Development and application of a high-performance liquid chromatography method using monolithic columns for the analysis of ecstasy tablets

Kim Mc Fadden; John Gillespie; Brian Carney; Daniel O'Driscoll

doi:10.1016/j.chroma.2006.01.071

Development and application of a high-performance liquid chromatography method using monolithic columns for the analysis of ecstasy tablets

Kim Mc Fadden, John Gillespie, Brian Carney, Daniel O'Driscoll

Department of Life & Physical Sciences

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

A rapid and selective HPLC method using monolithic columns was developed for the separation and quantification of the principal amphetamines in ecstasy tablets. Three monolithic (Chromolith RP18e) columns of different lengths (25, 50 and 100 mm) were assessed. Validation studies including linearity, selectivity, precision, accuracy and limit of detection and quantification were carried out using the Chromolith SpeedROD, RP-18e, 50 mm × 4.6 mm column. Column backpressure and van Deemter plots demonstrated that monolithic columns provide higher efficiency at higher flow rates when compared to particulate columns without the loss of peak resolution. Application of the monolithic column to a large number of ecstasy tablets seized in Ireland ensured its suitability for the routine analysis of ecstasy tablets.

Original language	English
Pages (from-to)	54-60
Number of pages	7
Journal	Journal of Chromatography A
Volume	1120
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2006.01.071
Publication status	Published - 7 Jul 2006

Keywords

Ecstasy tablets
HPLC
Monolithic columns

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10.1016/j.chroma.2006.01.071

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abstract = "A rapid and selective HPLC method using monolithic columns was developed for the separation and quantification of the principal amphetamines in ecstasy tablets. Three monolithic (Chromolith RP18e) columns of different lengths (25, 50 and 100 mm) were assessed. Validation studies including linearity, selectivity, precision, accuracy and limit of detection and quantification were carried out using the Chromolith SpeedROD, RP-18e, 50 mm × 4.6 mm column. Column backpressure and van Deemter plots demonstrated that monolithic columns provide higher efficiency at higher flow rates when compared to particulate columns without the loss of peak resolution. Application of the monolithic column to a large number of ecstasy tablets seized in Ireland ensured its suitability for the routine analysis of ecstasy tablets.",

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AU - Carney, Brian

AU - O'Driscoll, Daniel

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AB - A rapid and selective HPLC method using monolithic columns was developed for the separation and quantification of the principal amphetamines in ecstasy tablets. Three monolithic (Chromolith RP18e) columns of different lengths (25, 50 and 100 mm) were assessed. Validation studies including linearity, selectivity, precision, accuracy and limit of detection and quantification were carried out using the Chromolith SpeedROD, RP-18e, 50 mm × 4.6 mm column. Column backpressure and van Deemter plots demonstrated that monolithic columns provide higher efficiency at higher flow rates when compared to particulate columns without the loss of peak resolution. Application of the monolithic column to a large number of ecstasy tablets seized in Ireland ensured its suitability for the routine analysis of ecstasy tablets.

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Development and application of a high-performance liquid chromatography method using monolithic columns for the analysis of ecstasy tablets

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Keywords

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