Development of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments

K. Y. Kavanagh, J. E. Walsh, J. Murphy, M. Harmey, M. A. Farrell, O. Hardimann, R. Perryman

Research output: Contribution to journalConference articlepeer-review

5 Citations (Scopus)

Abstract

The continuing demand for non-invasive tools for use in clinical diagnosis has created the need for flexible and innovative optical systems which satisfy current requirements. We report the development of a microspectrophotometer system for use on mitochondrial respiratory pigments. This novel optical fibre set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Preliminary data is presented for the reduction of cytochrome-c by two methods. In the first, cytochrome-c was reduced in isolation using sodium dithionite. The second was an in-vivo simulation of the reduction of cytochrome-c using the mitochondrial extract from rat liver. The key features of the system are; front end adaptability, high sensitivity and fast scanning capabilities which are essential for the rapid biological reactions which are observed.

Original languageEnglish
Pages (from-to)88-95
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3105
DOIs
Publication statusPublished - 1997
Externally publishedYes
EventChemical, Biochemical and Environmental Fiber Senors IX - Munich, Germany
Duration: 16 Jun 199718 Jun 1997

Keywords

  • Cytochrome-c
  • Fibre optic sensor
  • Microspectrophotometry
  • Mitochondrial myopathies
  • Redox electron carriers

Fingerprint

Dive into the research topics of 'Development of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments'. Together they form a unique fingerprint.

Cite this