TY - JOUR
T1 - Enhancement in Bacterial Cellulolytic Enzyme Production Using Acid-Pretreated Banana Peel Waste
T2 - A Comparative Evaluation
AU - Khan, Pathan Ahemad
AU - Lal, Basant
AU - Singh, Preeti
AU - Singh, Rajeev
AU - Syed, Asad
AU - Elgorban, Abdallah M.
AU - Verma, Meenakshi
AU - Mishra, P. K.
AU - Verma, Bhawna
AU - Srivastava, Neha
AU - O’Donovan, Anthonia
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - Banana peel waste is one of the major contributors in the issue raised from solid waste, however, it can be valorized effectively due to high content of cellulose and hemicellulose. Significant conversion of banana waste includes cellulolytic enzymes and bioenergy production. In the present study, bacterial cellulase was produced using raw banana peel and ripe banana peel under SSF. Additionally, impact of acid pretreatment was investigated as one of strategy to improve cellulolytic enzyme production. A comparative evaluation of raw and ripe banana peels showed that ripe banana peels showed better enzyme production after pretreatment with 0.5% dilute HCl acid. In the series of enhancement of the enzyme production, temperature and pH of the SSF medium were also investigated, and found temperature 35 °C and pH 6.0 were optimum to produce maximum 3.5-U/ml FPA, 39-U/ml BGL, and 54-U/ml EG in 18-h SSF incubation. The study presented eco-friendly waste management to produce industrial enzyme for its promising application in waste valorization and biorefinery area.
AB - Banana peel waste is one of the major contributors in the issue raised from solid waste, however, it can be valorized effectively due to high content of cellulose and hemicellulose. Significant conversion of banana waste includes cellulolytic enzymes and bioenergy production. In the present study, bacterial cellulase was produced using raw banana peel and ripe banana peel under SSF. Additionally, impact of acid pretreatment was investigated as one of strategy to improve cellulolytic enzyme production. A comparative evaluation of raw and ripe banana peels showed that ripe banana peels showed better enzyme production after pretreatment with 0.5% dilute HCl acid. In the series of enhancement of the enzyme production, temperature and pH of the SSF medium were also investigated, and found temperature 35 °C and pH 6.0 were optimum to produce maximum 3.5-U/ml FPA, 39-U/ml BGL, and 54-U/ml EG in 18-h SSF incubation. The study presented eco-friendly waste management to produce industrial enzyme for its promising application in waste valorization and biorefinery area.
KW - Bacterial cellulase
KW - Banana peel waste
KW - Bioconversion
KW - SSF
KW - Waste management
UR - http://www.scopus.com/inward/record.url?scp=85167695133&partnerID=8YFLogxK
U2 - 10.1007/s12033-023-00816-w
DO - 10.1007/s12033-023-00816-w
M3 - Article
C2 - 37561276
AN - SCOPUS:85167695133
SN - 1073-6085
JO - Molecular Biotechnology
JF - Molecular Biotechnology
ER -
