@inbook{4660ffa1e9a84117ade3e2ea312ca5d8,
title = "Gene Expression Analysis by Reverse Transcription Quantitative PCR",
abstract = "The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a rapid detection technology that allows the amplification and quantification of specific RNA transcripts. RT-qPCR has increasingly been adopted for the detection and quantification of H. pylori across a range of sample types and applications. In addition, it is widely used to monitor host gene expression in cells and tissues in response to H. pylori infection. Outlined here is a two-step protocol that can be employed to analyze gene expression in H. pylori or H. pylori-infected samples.",
keywords = "RNA, Real-time PCR, Relative quantification, Reverse transcription, cDNA",
author = "Campion, {Eva M.} and Loughran, {Sin{\'e}ad T.}",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1302-3_8",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "61--74",
booktitle = "Methods in Molecular Biology",
}