In vitro protocols for measuring the antioxidant capacity of algal extracts

Owen Kenny, Nigel P. Brunton, Thomas J. Smyth

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

In the last decade a large amount of research has been directed at targeting algal resources for biologically active molecules. High-throughput in vitro antioxidant assays are routinely used to screen for biologically active compounds present in algal extracts when the requirement is to identify samples for progression to more detailed biological scrutiny. Whilst a myriad of antioxidant assays have been developed, this present chapter aims to give step-by-step practical guidance on how to carry out some of the most popular and biologically relevant assays at the bench.

Original languageEnglish
Pages (from-to)375-402
Number of pages28
JournalMethods in Molecular Biology
Volume1308
DOIs
Publication statusPublished - 2015

Keywords

  • Algal extracts
  • Antioxidants
  • Fluorescence assays
  • Preventative oxidation assays
  • Radical scavenging assays
  • Redox assays

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