Invariant Natural Killer T-cell anergy to endogenous myelin acetyl-glycolipids in multiple sclerosis

Carol M. Gately; Maria Podbielska; Timothy Counihan; Michael Hennessy; Teresa Leahy; Anthony P. Moran; Edward L. Hogan; Joan O'Keeffe

doi:10.1016/j.jneuroim.2013.02.020

Invariant Natural Killer T-cell anergy to endogenous myelin acetyl-glycolipids in multiple sclerosis

Carol M. Gately, Maria Podbielska, Timothy Counihan, Michael Hennessy, Teresa Leahy, Anthony P. Moran, Edward L. Hogan, Joan O'Keeffe

Department of Biopharmaceutical & Medical Science

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

To extend our studies on glycolipid-reactive invariant Natural Killer T-cell (iNKT-cell) function in multiple sclerosis (MS), we investigated the stimulatory activities of two myelin-derived glycolipids that are poly-acetylated derivatives of β-galactosylceramide designated as fast-migrating cerebrosides (FMC) by thin-layer chromatography. In healthy subjects, FMC stimulation of peripheral blood cells significantly expanded iNKT-cells similar to α-GalCer and induced significant increases in Th1, Th2 and Th17 cytokines. In marked contrast, MS patients failed to respond to FMCs or to α-GalCer stimulation indicating an anergic response. We propose that myelin-derived FMC glycolipids stimulate iNKT-cell responses in vivo and this is blocked in MS.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Journal of Neuroimmunology
Volume	259
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2013.02.020
Publication status	Published - 15 Jun 2013

Keywords

Cytokines
Flow cytometry
Multiple sclerosis
Myelin-derived glycolipids
Natural Killer T-cells

Access to Document

10.1016/j.jneuroim.2013.02.020

Cite this

@article{582979f144b844cbb85c1ca3487e19b6,

title = "Invariant Natural Killer T-cell anergy to endogenous myelin acetyl-glycolipids in multiple sclerosis",

abstract = "To extend our studies on glycolipid-reactive invariant Natural Killer T-cell (iNKT-cell) function in multiple sclerosis (MS), we investigated the stimulatory activities of two myelin-derived glycolipids that are poly-acetylated derivatives of β-galactosylceramide designated as fast-migrating cerebrosides (FMC) by thin-layer chromatography. In healthy subjects, FMC stimulation of peripheral blood cells significantly expanded iNKT-cells similar to α-GalCer and induced significant increases in Th1, Th2 and Th17 cytokines. In marked contrast, MS patients failed to respond to FMCs or to α-GalCer stimulation indicating an anergic response. We propose that myelin-derived FMC glycolipids stimulate iNKT-cell responses in vivo and this is blocked in MS.",

keywords = "Cytokines, Flow cytometry, Multiple sclerosis, Myelin-derived glycolipids, Natural Killer T-cells",

author = "Gately, {Carol M.} and Maria Podbielska and Timothy Counihan and Michael Hennessy and Teresa Leahy and Moran, {Anthony P.} and Hogan, {Edward L.} and Joan O'Keeffe",

year = "2013",

month = jun,

day = "15",

doi = "10.1016/j.jneuroim.2013.02.020",

language = "English",

volume = "259",

pages = "1--7",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

number = "1-2",

}

TY - JOUR

T1 - Invariant Natural Killer T-cell anergy to endogenous myelin acetyl-glycolipids in multiple sclerosis

AU - Gately, Carol M.

AU - Podbielska, Maria

AU - Counihan, Timothy

AU - Hennessy, Michael

AU - Leahy, Teresa

AU - Moran, Anthony P.

AU - Hogan, Edward L.

AU - O'Keeffe, Joan

PY - 2013/6/15

Y1 - 2013/6/15

N2 - To extend our studies on glycolipid-reactive invariant Natural Killer T-cell (iNKT-cell) function in multiple sclerosis (MS), we investigated the stimulatory activities of two myelin-derived glycolipids that are poly-acetylated derivatives of β-galactosylceramide designated as fast-migrating cerebrosides (FMC) by thin-layer chromatography. In healthy subjects, FMC stimulation of peripheral blood cells significantly expanded iNKT-cells similar to α-GalCer and induced significant increases in Th1, Th2 and Th17 cytokines. In marked contrast, MS patients failed to respond to FMCs or to α-GalCer stimulation indicating an anergic response. We propose that myelin-derived FMC glycolipids stimulate iNKT-cell responses in vivo and this is blocked in MS.

AB - To extend our studies on glycolipid-reactive invariant Natural Killer T-cell (iNKT-cell) function in multiple sclerosis (MS), we investigated the stimulatory activities of two myelin-derived glycolipids that are poly-acetylated derivatives of β-galactosylceramide designated as fast-migrating cerebrosides (FMC) by thin-layer chromatography. In healthy subjects, FMC stimulation of peripheral blood cells significantly expanded iNKT-cells similar to α-GalCer and induced significant increases in Th1, Th2 and Th17 cytokines. In marked contrast, MS patients failed to respond to FMCs or to α-GalCer stimulation indicating an anergic response. We propose that myelin-derived FMC glycolipids stimulate iNKT-cell responses in vivo and this is blocked in MS.

KW - Cytokines

KW - Flow cytometry

KW - Multiple sclerosis

KW - Myelin-derived glycolipids

KW - Natural Killer T-cells

UR - http://www.scopus.com/inward/record.url?scp=84877631908&partnerID=8YFLogxK

U2 - 10.1016/j.jneuroim.2013.02.020

DO - 10.1016/j.jneuroim.2013.02.020

M3 - Article

C2 - 23537888

AN - SCOPUS:84877631908

SN - 0165-5728

VL - 259

SP - 1

EP - 7

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

Invariant Natural Killer T-cell anergy to endogenous myelin acetyl-glycolipids in multiple sclerosis

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this