TY - JOUR
T1 - Isolation of Extracellular Vesicles From Microalgae
T2 - A Renewable and Scalable Bioprocess
AU - Paterna, Angela
AU - Rao, Estella
AU - Adamo, Giorgia
AU - Raccosta, Samuele
AU - Picciotto, Sabrina
AU - Romancino, Daniele
AU - Noto, Rosina
AU - Touzet, Nicolas
AU - Bongiovanni, Antonella
AU - Manno, Mauro
N1 - Publisher Copyright:
Copyright © 2022 Paterna, Rao, Adamo, Raccosta, Picciotto, Romancino, Noto, Touzet, Bongiovanni and Manno.
PY - 2022/3/14
Y1 - 2022/3/14
N2 - Extracellular vesicles (EVs) play a crucial role as potent signal transducers among cells, with the potential to operate cross-species and cross-kingdom communication. Nanoalgosomes are a subtype of EVs recently identified and isolated from microalgae. Microalgae represent a natural bioresource with the capacity to produce several secondary metabolites with a broad range of biological activities and commercial applications. The present study highlights the upstream and downstream processes required for the scalable production of nanoalgosomes from cultures of the marine microalgae Tetraselmis chuii. Different technical parameters, protocols, and conditions were assessed to improve EVs isolation by tangential flow filtration (TFF), aiming to enhance sample purity and yield. The optimization of the overall bioprocess was enhanced by quality control checks operated through robust biophysical and biochemical characterizations. Further, we showed the possibility of recycling by TFF microalgae cells post-EVs isolation for multiple EV production cycles. The present results highlight the potential of nanoalgosome production as a scalable, cost-effective bioprocess suitable for diverse scientific and industrial exploitations.
AB - Extracellular vesicles (EVs) play a crucial role as potent signal transducers among cells, with the potential to operate cross-species and cross-kingdom communication. Nanoalgosomes are a subtype of EVs recently identified and isolated from microalgae. Microalgae represent a natural bioresource with the capacity to produce several secondary metabolites with a broad range of biological activities and commercial applications. The present study highlights the upstream and downstream processes required for the scalable production of nanoalgosomes from cultures of the marine microalgae Tetraselmis chuii. Different technical parameters, protocols, and conditions were assessed to improve EVs isolation by tangential flow filtration (TFF), aiming to enhance sample purity and yield. The optimization of the overall bioprocess was enhanced by quality control checks operated through robust biophysical and biochemical characterizations. Further, we showed the possibility of recycling by TFF microalgae cells post-EVs isolation for multiple EV production cycles. The present results highlight the potential of nanoalgosome production as a scalable, cost-effective bioprocess suitable for diverse scientific and industrial exploitations.
KW - extracellular vesicles
KW - microalgae
KW - nanoalgosomes
KW - renewable biosources
KW - tangential flow filtration
UR - http://www.scopus.com/inward/record.url?scp=85127404299&partnerID=8YFLogxK
U2 - 10.3389/fbioe.2022.836747
DO - 10.3389/fbioe.2022.836747
M3 - Article
AN - SCOPUS:85127404299
SN - 2296-4185
VL - 10
JO - Frontiers in Bioengineering and Biotechnology
JF - Frontiers in Bioengineering and Biotechnology
M1 - 836747
ER -
