TY - JOUR
T1 - Isolation of extracellular vesicles from microalgae
T2 - Towards the production of sustainable and natural nanocarriers of bioactive compounds
AU - Picciotto, Sabrina
AU - Barone, Maria E.
AU - Fierli, David
AU - Aranyos, Anita
AU - Adamo, Giorgia
AU - Božič, Darja
AU - Romancino, Daniele P.
AU - Stanly, Christopher
AU - Parkes, Rachel
AU - Morsbach, Svenja
AU - Raccosta, Samuele
AU - Paganini, Carolina
AU - Cusimano, Antonella
AU - Martorana, Vincenzo
AU - Noto, Rosina
AU - Carrotta, Rita
AU - Librizzi, Fabio
AU - Capasso Palmiero, Umberto
AU - Santonicola, Pamela
AU - Iglič, Ales
AU - Gai, Meiyu
AU - Corcuera, Laura
AU - Kisslinger, Annamaria
AU - Di Schiavi, Elia
AU - Landfester, Katharina
AU - Liguori, Giovanna L.
AU - Kralj-Iglič, Veronika
AU - Arosio, Paolo
AU - Pocsfalvi, Gabriella
AU - Manno, Mauro
AU - Touzet, Nicolas
AU - Bongiovanni, Antonella
N1 - Publisher Copyright:
© 2021 The Royal Society of Chemistry.
PY - 2021/4/21
Y1 - 2021/4/21
N2 - Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeutics, precision medicine and other biotechnology sectors. Novel bio-based nanotechnologies have recently arisen, which are based on the exploitation of extracellular vesicles (EVs). In this context, it has become essential to identify suitable organisms or cellular types to act as reliable sources of EVs and to develop their pilot- to large-scale production. The discovery of new biosources and the optimisation of related bioprocesses for the isolation and functionalisation of nano-delivery vehicles are fundamental to further develop therapeutic and biotechnological applications. Microalgae constitute sustainable sources of bioactive compounds with a range of sectorial applications including for example the formulation of health supplements, cosmetic products or food ingredients. In this study, we demonstrate that microalgae are promising producers of EVs. By analysing the nanosized extracellular nano-objects produced by eighteen microalgal species, we identified seven promising EV-producing strains belonging to distinct lineages, suggesting that the production of EVs in microalgae is an evolutionary conserved trait. Here we report the selection process and focus on one of this seven species, the glaucophyte Cyanophora paradoxa, which returned a protein yield in the small EV fraction of 1 μg of EV proteins per mg of dry weight of microalgal biomass (corresponding to 109 particles per mg of dried biomass) and EVs with a diameter of 130 nm (mode), as determined by the micro bicinchoninic acid assay, nanoparticle tracking and dynamic light scattering analyses. Moreover, the extracellular nanostructures isolated from the conditioned media of microalgae species returned positive immunoblot signals for some commonly used EV-biomarkers such as Alix, Enolase, HSP70, and β-actin. Overall, this work establishes a platform for the efficient production of EVs from a sustainable bioresource and highlights the potential of microalgal EVs as novel biogenic nanovehicles.
AB - Safe, efficient and specific nano-delivery systems are essential for current and emerging therapeutics, precision medicine and other biotechnology sectors. Novel bio-based nanotechnologies have recently arisen, which are based on the exploitation of extracellular vesicles (EVs). In this context, it has become essential to identify suitable organisms or cellular types to act as reliable sources of EVs and to develop their pilot- to large-scale production. The discovery of new biosources and the optimisation of related bioprocesses for the isolation and functionalisation of nano-delivery vehicles are fundamental to further develop therapeutic and biotechnological applications. Microalgae constitute sustainable sources of bioactive compounds with a range of sectorial applications including for example the formulation of health supplements, cosmetic products or food ingredients. In this study, we demonstrate that microalgae are promising producers of EVs. By analysing the nanosized extracellular nano-objects produced by eighteen microalgal species, we identified seven promising EV-producing strains belonging to distinct lineages, suggesting that the production of EVs in microalgae is an evolutionary conserved trait. Here we report the selection process and focus on one of this seven species, the glaucophyte Cyanophora paradoxa, which returned a protein yield in the small EV fraction of 1 μg of EV proteins per mg of dry weight of microalgal biomass (corresponding to 109 particles per mg of dried biomass) and EVs with a diameter of 130 nm (mode), as determined by the micro bicinchoninic acid assay, nanoparticle tracking and dynamic light scattering analyses. Moreover, the extracellular nanostructures isolated from the conditioned media of microalgae species returned positive immunoblot signals for some commonly used EV-biomarkers such as Alix, Enolase, HSP70, and β-actin. Overall, this work establishes a platform for the efficient production of EVs from a sustainable bioresource and highlights the potential of microalgal EVs as novel biogenic nanovehicles.
UR - http://www.scopus.com/inward/record.url?scp=85104577627&partnerID=8YFLogxK
U2 - 10.1039/d0bm01696a
DO - 10.1039/d0bm01696a
M3 - Article
C2 - 33620041
AN - SCOPUS:85104577627
SN - 2047-4830
VL - 9
SP - 2917
EP - 2930
JO - Biomaterials Science
JF - Biomaterials Science
IS - 8
ER -