Methylome of human skeletal muscle after acute & chronic resistance exercise training, detraining & retraining

R. A. Seaborne; J. Strauss; M. Cocks; S. Shepherd; T. D. O’brien; K. A. van Someren; P. G. Bell; C. Murgatroyd; J. P. Morton; C. E. Stewart; C. A. Mein; A. P. Sharples

doi:10.1038/sdata.2018.213

Methylome of human skeletal muscle after acute & chronic resistance exercise training, detraining & retraining

R. A. Seaborne, J. Strauss, M. Cocks, S. Shepherd, T. D. O’brien, K. A. van Someren, P. G. Bell, C. Murgatroyd, J. P. Morton, C. E. Stewart, C. A. Mein, A. P. Sharples

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

DNA methylation is an important epigenetic modification that can regulate gene expression following environmental encounters without changes to the genetic code. Using Infinium MethylationEPIC BeadChip Arrays (850,000 CpG sites) we analysed for the first time, DNA isolated from untrained human skeletal muscle biopsies (vastus lateralis) at baseline (rest) and immediately following an acute (single) bout of resistance exercise. In the same participants, we also analysed the methylome following a period of muscle growth (hypertrophy) evoked via chronic (repeated bouts-3 sessions/wk) resistance exercise (RE) (training) over 7-weeks, followed by complete exercise cessation for 7-weeks returning muscle back to baseline levels (detraining), and finally followed by a subsequent 7-week period of RE-induced hypertrophy (retraining). These valuable methylome data sets described in the present manuscript and deposited in an open-access repository can now be shared and re-used to enable the identification of epigenetically regulated genes/ networks that are modified after acute anabolic stimuli and hypertrophy, and further investigate the phenomenon of epigenetic memory in skeletal muscle.

Original language	English
Article number	180213
Journal	Scientific Data
Volume	5
DOIs	https://doi.org/10.1038/sdata.2018.213
Publication status	Published - 2018
Externally published	Yes

Access to Document

10.1038/sdata.2018.213

Cite this

Seaborne, R. A., Strauss, J., Cocks, M., Shepherd, S., O’brien, T. D., van Someren, K. A., Bell, P. G., Murgatroyd, C., Morton, J. P., Stewart, C. E., Mein, C. A., & Sharples, A. P. (2018). Methylome of human skeletal muscle after acute & chronic resistance exercise training, detraining & retraining. Scientific Data, 5, Article 180213. https://doi.org/10.1038/sdata.2018.213

@article{35ea5883ed5b4384bcb93082367ef739,

title = "Methylome of human skeletal muscle after acute \& chronic resistance exercise training, detraining \& retraining",

abstract = "DNA methylation is an important epigenetic modification that can regulate gene expression following environmental encounters without changes to the genetic code. Using Infinium MethylationEPIC BeadChip Arrays (850,000 CpG sites) we analysed for the first time, DNA isolated from untrained human skeletal muscle biopsies (vastus lateralis) at baseline (rest) and immediately following an acute (single) bout of resistance exercise. In the same participants, we also analysed the methylome following a period of muscle growth (hypertrophy) evoked via chronic (repeated bouts-3 sessions/wk) resistance exercise (RE) (training) over 7-weeks, followed by complete exercise cessation for 7-weeks returning muscle back to baseline levels (detraining), and finally followed by a subsequent 7-week period of RE-induced hypertrophy (retraining). These valuable methylome data sets described in the present manuscript and deposited in an open-access repository can now be shared and re-used to enable the identification of epigenetically regulated genes/ networks that are modified after acute anabolic stimuli and hypertrophy, and further investigate the phenomenon of epigenetic memory in skeletal muscle.",

author = "Seaborne, \{R. A.\} and J. Strauss and M. Cocks and S. Shepherd and O{\textquoteright}brien, \{T. D.\} and \{van Someren\}, \{K. A.\} and Bell, \{P. G.\} and C. Murgatroyd and Morton, \{J. P.\} and Stewart, \{C. E.\} and Mein, \{C. A.\} and Sharples, \{A. P.\}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2018.",

year = "2018",

doi = "10.1038/sdata.2018.213",

language = "English",

volume = "5",

journal = "Scientific Data",

issn = "2052-4463",

}

TY - JOUR

T1 - Methylome of human skeletal muscle after acute & chronic resistance exercise training, detraining & retraining

AU - Seaborne, R. A.

AU - Strauss, J.

AU - Cocks, M.

AU - Shepherd, S.

AU - O’brien, T. D.

AU - van Someren, K. A.

AU - Bell, P. G.

AU - Murgatroyd, C.

AU - Morton, J. P.

AU - Stewart, C. E.

AU - Mein, C. A.

AU - Sharples, A. P.

PY - 2018

Y1 - 2018

N2 - DNA methylation is an important epigenetic modification that can regulate gene expression following environmental encounters without changes to the genetic code. Using Infinium MethylationEPIC BeadChip Arrays (850,000 CpG sites) we analysed for the first time, DNA isolated from untrained human skeletal muscle biopsies (vastus lateralis) at baseline (rest) and immediately following an acute (single) bout of resistance exercise. In the same participants, we also analysed the methylome following a period of muscle growth (hypertrophy) evoked via chronic (repeated bouts-3 sessions/wk) resistance exercise (RE) (training) over 7-weeks, followed by complete exercise cessation for 7-weeks returning muscle back to baseline levels (detraining), and finally followed by a subsequent 7-week period of RE-induced hypertrophy (retraining). These valuable methylome data sets described in the present manuscript and deposited in an open-access repository can now be shared and re-used to enable the identification of epigenetically regulated genes/ networks that are modified after acute anabolic stimuli and hypertrophy, and further investigate the phenomenon of epigenetic memory in skeletal muscle.

AB - DNA methylation is an important epigenetic modification that can regulate gene expression following environmental encounters without changes to the genetic code. Using Infinium MethylationEPIC BeadChip Arrays (850,000 CpG sites) we analysed for the first time, DNA isolated from untrained human skeletal muscle biopsies (vastus lateralis) at baseline (rest) and immediately following an acute (single) bout of resistance exercise. In the same participants, we also analysed the methylome following a period of muscle growth (hypertrophy) evoked via chronic (repeated bouts-3 sessions/wk) resistance exercise (RE) (training) over 7-weeks, followed by complete exercise cessation for 7-weeks returning muscle back to baseline levels (detraining), and finally followed by a subsequent 7-week period of RE-induced hypertrophy (retraining). These valuable methylome data sets described in the present manuscript and deposited in an open-access repository can now be shared and re-used to enable the identification of epigenetically regulated genes/ networks that are modified after acute anabolic stimuli and hypertrophy, and further investigate the phenomenon of epigenetic memory in skeletal muscle.

UR - https://www.scopus.com/pages/publications/85055618593

U2 - 10.1038/sdata.2018.213

DO - 10.1038/sdata.2018.213

M3 - Article

C2 - 30375987

AN - SCOPUS:85055618593

SN - 2052-4463

VL - 5

JO - Scientific Data

JF - Scientific Data

M1 - 180213

ER -

Methylome of human skeletal muscle after acute & chronic resistance exercise training, detraining & retraining

Abstract

Access to Document

Other files and links

Fingerprint

Cite this