Optical sectioning using finite-sized, multiple aperture arrays in fluorescence direct-view microscopy; experimental results

P. J. Smith; C. Taylor; E. M. McCabe

Optical sectioning using finite-sized, multiple aperture arrays in fluorescence direct-view microscopy; experimental results

P. J. Smith
, C. Taylor
, E. M. McCabe

Trinity College Dublin

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Confocal fluorescence imaging is widely used, particularly for biological applications, and also notably in direct-view microscopes. Recent work has compared the use of coherent and incoherent illumination sources on the optical sectioning characteristics of fluorescence direct-view microscopes. However this detailed comparison has been done in theory. This paper addresses the experimental aspects of using coherent light sources in fluorescence imaging using a range of finite-sized, multiple-aperture arrays. The experimental difficulties of choosing a suitable uniform, flat, fluorescent plane with a high quantum efficiency are considered. Axial response curves obtained with a fluorescent laser dye sample are presented.

Original language	English
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Publisher	Society of Photo-Optical Instrumentation Engineers
Pages	236-240
Number of pages	5
ISBN (Print)	0819430757
Publication status	Published - 1999
Externally published	Yes
Event	Proceedings of the 1999 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI - San Jose, CA, USA Duration: 24 Jan 1999 → 25 Jan 1999

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3605
ISSN (Print)	0277-786X

Conference

Conference	Proceedings of the 1999 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI
City	San Jose, CA, USA
Period	24/01/99 → 25/01/99

Cite this

Smith, P. J., Taylor, C., & McCabe, E. M. (1999). Optical sectioning using finite-sized, multiple aperture arrays in fluorescence direct-view microscopy; experimental results. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 236-240). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 3605). Society of Photo-Optical Instrumentation Engineers.

Smith, P. J. ; Taylor, C. ; McCabe, E. M. / Optical sectioning using finite-sized, multiple aperture arrays in fluorescence direct-view microscopy; experimental results. Proceedings of SPIE - The International Society for Optical Engineering. Society of Photo-Optical Instrumentation Engineers, 1999. pp. 236-240 (Proceedings of SPIE - The International Society for Optical Engineering).

@inproceedings{3aa02581c2884afbadf3e0bbd78626fd,

title = "Optical sectioning using finite-sized, multiple aperture arrays in fluorescence direct-view microscopy; experimental results",

abstract = "Confocal fluorescence imaging is widely used, particularly for biological applications, and also notably in direct-view microscopes. Recent work has compared the use of coherent and incoherent illumination sources on the optical sectioning characteristics of fluorescence direct-view microscopes. However this detailed comparison has been done in theory. This paper addresses the experimental aspects of using coherent light sources in fluorescence imaging using a range of finite-sized, multiple-aperture arrays. The experimental difficulties of choosing a suitable uniform, flat, fluorescent plane with a high quantum efficiency are considered. Axial response curves obtained with a fluorescent laser dye sample are presented.",

author = "Smith, \{P. J.\} and C. Taylor and McCabe, \{E. M.\}",

year = "1999",

language = "English",

isbn = "0819430757",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "Society of Photo-Optical Instrumentation Engineers",

pages = "236--240",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

note = "Proceedings of the 1999 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI ; Conference date: 24-01-1999 Through 25-01-1999",

}

Smith, PJ, Taylor, C & McCabe, EM 1999, Optical sectioning using finite-sized, multiple aperture arrays in fluorescence direct-view microscopy; experimental results. in Proceedings of SPIE - The International Society for Optical Engineering. Proceedings of SPIE - The International Society for Optical Engineering, vol. 3605, Society of Photo-Optical Instrumentation Engineers, pp. 236-240, Proceedings of the 1999 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI, San Jose, CA, USA, 24/01/99.

Optical sectioning using finite-sized, multiple aperture arrays in fluorescence direct-view microscopy; experimental results. / Smith, P. J.; Taylor, C.; McCabe, E. M.
Proceedings of SPIE - The International Society for Optical Engineering. Society of Photo-Optical Instrumentation Engineers, 1999. p. 236-240 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 3605).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Optical sectioning using finite-sized, multiple aperture arrays in fluorescence direct-view microscopy; experimental results

AU - Smith, P. J.

AU - Taylor, C.

AU - McCabe, E. M.

PY - 1999

Y1 - 1999

N2 - Confocal fluorescence imaging is widely used, particularly for biological applications, and also notably in direct-view microscopes. Recent work has compared the use of coherent and incoherent illumination sources on the optical sectioning characteristics of fluorescence direct-view microscopes. However this detailed comparison has been done in theory. This paper addresses the experimental aspects of using coherent light sources in fluorescence imaging using a range of finite-sized, multiple-aperture arrays. The experimental difficulties of choosing a suitable uniform, flat, fluorescent plane with a high quantum efficiency are considered. Axial response curves obtained with a fluorescent laser dye sample are presented.

AB - Confocal fluorescence imaging is widely used, particularly for biological applications, and also notably in direct-view microscopes. Recent work has compared the use of coherent and incoherent illumination sources on the optical sectioning characteristics of fluorescence direct-view microscopes. However this detailed comparison has been done in theory. This paper addresses the experimental aspects of using coherent light sources in fluorescence imaging using a range of finite-sized, multiple-aperture arrays. The experimental difficulties of choosing a suitable uniform, flat, fluorescent plane with a high quantum efficiency are considered. Axial response curves obtained with a fluorescent laser dye sample are presented.

UR - https://www.scopus.com/pages/publications/0032684895

M3 - Conference contribution

AN - SCOPUS:0032684895

SN - 0819430757

T3 - Proceedings of SPIE - The International Society for Optical Engineering

SP - 236

EP - 240

BT - Proceedings of SPIE - The International Society for Optical Engineering

PB - Society of Photo-Optical Instrumentation Engineers

T2 - Proceedings of the 1999 Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing VI

Y2 - 24 January 1999 through 25 January 1999

ER -

Smith PJ, Taylor C, McCabe EM. Optical sectioning using finite-sized, multiple aperture arrays in fluorescence direct-view microscopy; experimental results. In Proceedings of SPIE - The International Society for Optical Engineering. Society of Photo-Optical Instrumentation Engineers. 1999. p. 236-240. (Proceedings of SPIE - The International Society for Optical Engineering).

Optical sectioning using finite-sized, multiple aperture arrays in fluorescence direct-view microscopy; experimental results

Abstract

Publication series

Conference

Other files and links

Fingerprint

Cite this