Phloroglucinols from anti-microbial deposit-resins of Australian stingless bees (Tetragonula carbonaria)

Carmelina Flavia Massaro; Thomas J. Smyth; W. Franklin Smyth; Tim Heard; Sara D. Leonhardt; Mohammad Katouli; Helen M. Wallace; Peter Brooks

doi:10.1002/ptr.5225

Phloroglucinols from anti-microbial deposit-resins of Australian stingless bees (Tetragonula carbonaria)

Carmelina Flavia Massaro, Thomas J. Smyth, W. Franklin Smyth, Tim Heard, Sara D. Leonhardt, Mohammad Katouli, Helen M. Wallace, Peter Brooks

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Stingless bees accumulate deposits of plant resins that are mixed with beeswax to produce propolis. Previous studies have reported anti-microbial constituents of stingless bee (Tetragonula carbonaria) propolis from East Australia, but several components remained to be characterized. In the search of natural products yet unreported for Australian propolis, four bee deposit-resins of T. carbonaria bees were analysed by gas and liquid chromatography mass spectrometry with accurate mass measurements. Ethanolic extracts of the deposit-resins were tested in vitro against Staphylococcus aureus ATCC 25983 and Pseudomonas aeruginosa ATCC 27853 by the agar diffusion method. Phloroglucinols, flavonoids and isoprenoids were identified in samples. The crude extracts showed strong anti-staphylococcal effects but were less active against the Gram-negative bacterium. The diagnostic data enabled the identification of markers that can be used for profiling other Australian propolis sources and to target the isolation of bioactive phloroglucinols in future studies against antibiotic resistant S. aureus strains.

Original language	English
Pages (from-to)	48-58
Number of pages	11
Journal	Phytotherapy Research
Volume	29
Issue number	1
DOIs	https://doi.org/10.1002/ptr.5225
Publication status	Published - Jan 2015
Externally published	Yes

Keywords

Dereplication
Flavonoids
Myrtaceae
Propolis
Staphylococcus aureus
Triketones

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10.1002/ptr.5225

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title = "Phloroglucinols from anti-microbial deposit-resins of Australian stingless bees (Tetragonula carbonaria)",

abstract = "Stingless bees accumulate deposits of plant resins that are mixed with beeswax to produce propolis. Previous studies have reported anti-microbial constituents of stingless bee (Tetragonula carbonaria) propolis from East Australia, but several components remained to be characterized. In the search of natural products yet unreported for Australian propolis, four bee deposit-resins of T. carbonaria bees were analysed by gas and liquid chromatography mass spectrometry with accurate mass measurements. Ethanolic extracts of the deposit-resins were tested in vitro against Staphylococcus aureus ATCC 25983 and Pseudomonas aeruginosa ATCC 27853 by the agar diffusion method. Phloroglucinols, flavonoids and isoprenoids were identified in samples. The crude extracts showed strong anti-staphylococcal effects but were less active against the Gram-negative bacterium. The diagnostic data enabled the identification of markers that can be used for profiling other Australian propolis sources and to target the isolation of bioactive phloroglucinols in future studies against antibiotic resistant S. aureus strains.",

keywords = "Dereplication, Flavonoids, Myrtaceae, Propolis, Staphylococcus aureus, Triketones",

author = "Massaro, {Carmelina Flavia} and Smyth, {Thomas J.} and Smyth, {W. Franklin} and Tim Heard and Leonhardt, {Sara D.} and Mohammad Katouli and Wallace, {Helen M.} and Peter Brooks",

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year = "2015",

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doi = "10.1002/ptr.5225",

language = "English",

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AU - Massaro, Carmelina Flavia

AU - Smyth, Thomas J.

AU - Smyth, W. Franklin

AU - Heard, Tim

AU - Leonhardt, Sara D.

AU - Katouli, Mohammad

AU - Wallace, Helen M.

AU - Brooks, Peter

PY - 2015/1

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N2 - Stingless bees accumulate deposits of plant resins that are mixed with beeswax to produce propolis. Previous studies have reported anti-microbial constituents of stingless bee (Tetragonula carbonaria) propolis from East Australia, but several components remained to be characterized. In the search of natural products yet unreported for Australian propolis, four bee deposit-resins of T. carbonaria bees were analysed by gas and liquid chromatography mass spectrometry with accurate mass measurements. Ethanolic extracts of the deposit-resins were tested in vitro against Staphylococcus aureus ATCC 25983 and Pseudomonas aeruginosa ATCC 27853 by the agar diffusion method. Phloroglucinols, flavonoids and isoprenoids were identified in samples. The crude extracts showed strong anti-staphylococcal effects but were less active against the Gram-negative bacterium. The diagnostic data enabled the identification of markers that can be used for profiling other Australian propolis sources and to target the isolation of bioactive phloroglucinols in future studies against antibiotic resistant S. aureus strains.

AB - Stingless bees accumulate deposits of plant resins that are mixed with beeswax to produce propolis. Previous studies have reported anti-microbial constituents of stingless bee (Tetragonula carbonaria) propolis from East Australia, but several components remained to be characterized. In the search of natural products yet unreported for Australian propolis, four bee deposit-resins of T. carbonaria bees were analysed by gas and liquid chromatography mass spectrometry with accurate mass measurements. Ethanolic extracts of the deposit-resins were tested in vitro against Staphylococcus aureus ATCC 25983 and Pseudomonas aeruginosa ATCC 27853 by the agar diffusion method. Phloroglucinols, flavonoids and isoprenoids were identified in samples. The crude extracts showed strong anti-staphylococcal effects but were less active against the Gram-negative bacterium. The diagnostic data enabled the identification of markers that can be used for profiling other Australian propolis sources and to target the isolation of bioactive phloroglucinols in future studies against antibiotic resistant S. aureus strains.

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KW - Flavonoids

KW - Myrtaceae

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KW - Staphylococcus aureus

KW - Triketones

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Phloroglucinols from anti-microbial deposit-resins of Australian stingless bees (Tetragonula carbonaria)

Abstract

Keywords

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