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Protein quantitation and analysis of purity

    • Dundalk Institute of Technology
    • Dublin City University

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    19 Citations (Scopus)

    Abstract

    The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and is a critical factor in many clinical applications. The large number and variety of techniques employed for this purpose is therefore not surprising. The selection of a suitable assay is dependent on such factors as the level of sensitivity required, the presence of interfering agents, and the composition of the protein itself. Here, protocols for the most commonly used protein determination methodologies are outlined, as well as for the more recently adapted technique of quantitative immuno-Polymerase Chain Reaction.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages225-255
    Number of pages31
    DOIs
    Publication statusPublished - 2017

    Publication series

    NameMethods in Molecular Biology
    Volume1485
    ISSN (Print)1064-3745

    Keywords

    • BCA
    • Bradford
    • ELISA
    • Lowry
    • Protein
    • Quantitative immuno-PCR (qIPCR)

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