Protein Quantitation and Analysis of Purity

Eva M. Campion, Sinéad T. Loughran, Dermot Walls

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

32 Citations (Scopus)

Abstract

The accurate quantitation of proteins and an analysis of their purity are essential in numerous areas of scientific research, and are a critical factor in many clinical applications. The large and varied number of techniques employed for this purpose is therefore not surprising. The selection of a suitable assay is dependent on factors such as the level of sensitivity required, the presence of interfering agents, and the composition of the protein itself. Here, protocols for the most commonly used protein determination methodologies are outlined, as well as for the more recently adapted technique of quantitative immuno-polymerase chain reaction.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages229-258
Number of pages30
DOIs
Publication statusPublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume681
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • BCA
  • Bradford
  • Lowry
  • Protein
  • Quantitative immuno-PCR (qIPCR)

Fingerprint

Dive into the research topics of 'Protein Quantitation and Analysis of Purity'. Together they form a unique fingerprint.

Cite this