TY - JOUR
T1 - Quantitative UPLC-MS/MS analysis of chlorogenic acid derivatives in antioxidant fractionates from dandelion (Taraxacum officinale) root
AU - Kenny, Owen
AU - Smyth, Thomas J.
AU - Hewage, Chandralal M.
AU - Brunton, Nigel P.
N1 - Publisher Copyright:
© 2014 Institute of Food Science and Technology.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - Summary: While qualitative studies have identified chlorogenic acids in antioxidant extracts, particularly ethyl acetate-derived extracts, of Taraxacum officinale, quantitative analysis of these phenolic compounds remains largely unreported for this species. In this study, bioactivity-guided fractionation of an antioxidant crude ethyl acetate extract (DPPH = 295.481 ± 0.955 mg TE g-1 extract) from T. officinale root resulted in a number of reverse-phase fractions that demonstrated high antioxidant activity (DPPH = 1058.733-1312.136 mg TE g-1 extract), stronger than that of the synthetic antioxidant Trolox®. UPLC-MS/MS screening of these fractions for the presence of selected mono- and di-caffeoylquinic acids revealed large quantities of 1,5-dicaffeoylquinic acid present in several fractions (853.052-907.324 μg mg-1), respectively. Due to the antioxidant potency and high levels of 1,5-dicaffeoylquinic acid observed in these fractions, it was concluded that specifically this chlorogenic acid derivative is a major contributor to the antioxidant efficacy of dandelion root.
AB - Summary: While qualitative studies have identified chlorogenic acids in antioxidant extracts, particularly ethyl acetate-derived extracts, of Taraxacum officinale, quantitative analysis of these phenolic compounds remains largely unreported for this species. In this study, bioactivity-guided fractionation of an antioxidant crude ethyl acetate extract (DPPH = 295.481 ± 0.955 mg TE g-1 extract) from T. officinale root resulted in a number of reverse-phase fractions that demonstrated high antioxidant activity (DPPH = 1058.733-1312.136 mg TE g-1 extract), stronger than that of the synthetic antioxidant Trolox®. UPLC-MS/MS screening of these fractions for the presence of selected mono- and di-caffeoylquinic acids revealed large quantities of 1,5-dicaffeoylquinic acid present in several fractions (853.052-907.324 μg mg-1), respectively. Due to the antioxidant potency and high levels of 1,5-dicaffeoylquinic acid observed in these fractions, it was concluded that specifically this chlorogenic acid derivative is a major contributor to the antioxidant efficacy of dandelion root.
KW - Antioxidant activity
KW - Bioactivity-guided fractionation
KW - Caffeoylquinic acids
KW - Chlorogenic acid
KW - Taraxacum officinale
KW - UPLC-MS/MS
UR - http://www.scopus.com/inward/record.url?scp=84923084161&partnerID=8YFLogxK
U2 - 10.1111/ijfs.12668
DO - 10.1111/ijfs.12668
M3 - Article
AN - SCOPUS:84923084161
SN - 0950-5423
VL - 50
SP - 766
EP - 773
JO - International Journal of Food Science and Technology
JF - International Journal of Food Science and Technology
IS - 3
ER -