Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes

K. Y. Kavanagh; J. E. Walsh; J. Murphy; M. Harmey; M. A. Farrell; O. Hardimann; R. Perryman

doi:10.1117/12.308038

Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes

K. Y. Kavanagh, J. E. Walsh, J. Murphy, M. Harmey, M. A. Farrell, O. Hardimann, R. Perryman

Dublin Institute of Technology

Research output: Contribution to journal › Conference article › peer-review

2 Citations (Scopus)

Abstract

We report the development of a microspectrophotometer system for use on micro samples of mitochondrial respiratory pigments. A novel optical fibre set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Data is presented for the reduction of cytochrome-c and for the effect of temperature on the levels of complex II/III activity from the mitochondria of rat liver. This in-vivo simulation of the reduction of cytochrome-c can be observed using a fibre optic probe which requires less than twenty μl of sample for analysis. The key features of the, system are; front end adaptability, high sensitivity and fast multispectral acquisition which are essential for the biological reactions which are observed.

Original language	English
Pages (from-to)	258-265
Number of pages	8
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3253
DOIs	https://doi.org/10.1117/12.308038
Publication status	Published - 1998
Externally published	Yes
Event	Biomedical Sensing and Imaging Technologies - San Jose, CA, United States Duration: 26 Jan 1998 → 27 Jan 1998

Keywords

Cytochrome-c
Dip-probe
Fibre optics
Microspectrophotometry
Mitochondrial myopathies
Redox electron carriers
Temperature control

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10.1117/12.308038

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title = "Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes",

abstract = "We report the development of a microspectrophotometer system for use on micro samples of mitochondrial respiratory pigments. A novel optical fibre set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Data is presented for the reduction of cytochrome-c and for the effect of temperature on the levels of complex II/III activity from the mitochondria of rat liver. This in-vivo simulation of the reduction of cytochrome-c can be observed using a fibre optic probe which requires less than twenty μl of sample for analysis. The key features of the, system are; front end adaptability, high sensitivity and fast multispectral acquisition which are essential for the biological reactions which are observed.",

keywords = "Cytochrome-c, Dip-probe, Fibre optics, Microspectrophotometry, Mitochondrial myopathies, Redox electron carriers, Temperature control",

author = "Kavanagh, {K. Y.} and Walsh, {J. E.} and J. Murphy and M. Harmey and Farrell, {M. A.} and O. Hardimann and R. Perryman",

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doi = "10.1117/12.308038",

language = "English",

volume = "3253",

pages = "258--265",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

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note = "Biomedical Sensing and Imaging Technologies ; Conference date: 26-01-1998 Through 27-01-1998",

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TY - JOUR

T1 - Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes

AU - Kavanagh, K. Y.

AU - Walsh, J. E.

AU - Murphy, J.

AU - Harmey, M.

AU - Farrell, M. A.

AU - Hardimann, O.

AU - Perryman, R.

PY - 1998

Y1 - 1998

N2 - We report the development of a microspectrophotometer system for use on micro samples of mitochondrial respiratory pigments. A novel optical fibre set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Data is presented for the reduction of cytochrome-c and for the effect of temperature on the levels of complex II/III activity from the mitochondria of rat liver. This in-vivo simulation of the reduction of cytochrome-c can be observed using a fibre optic probe which requires less than twenty μl of sample for analysis. The key features of the, system are; front end adaptability, high sensitivity and fast multispectral acquisition which are essential for the biological reactions which are observed.

AB - We report the development of a microspectrophotometer system for use on micro samples of mitochondrial respiratory pigments. A novel optical fibre set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Data is presented for the reduction of cytochrome-c and for the effect of temperature on the levels of complex II/III activity from the mitochondria of rat liver. This in-vivo simulation of the reduction of cytochrome-c can be observed using a fibre optic probe which requires less than twenty μl of sample for analysis. The key features of the, system are; front end adaptability, high sensitivity and fast multispectral acquisition which are essential for the biological reactions which are observed.

KW - Cytochrome-c

KW - Dip-probe

KW - Fibre optics

KW - Microspectrophotometry

KW - Mitochondrial myopathies

KW - Redox electron carriers

KW - Temperature control

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U2 - 10.1117/12.308038

DO - 10.1117/12.308038

M3 - Conference article

AN - SCOPUS:0032223868

SN - 0277-786X

VL - 3253

SP - 258

EP - 265

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Biomedical Sensing and Imaging Technologies

Y2 - 26 January 1998 through 27 January 1998

ER -

Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes

Abstract

Keywords

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