Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes

K. Y. Kavanagh; J. E. Walsh; J. Murphy; M. Harmey; M. A. Farrell; O. Hardimann; R. Perryman

doi:10.1117/12.308038

Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes

K. Y. Kavanagh
, J. E. Walsh
, J. Murphy
, M. Harmey
, M. A. Farrell
, O. Hardimann
, R. Perryman

Dublin Institute of Technology

Research output: Contribution to journal › Conference article › peer-review

2 Citations (Scopus)

Abstract

We report the development of a microspectrophotometer system for use on micro samples of mitochondrial respiratory pigments. A novel optical fibre set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Data is presented for the reduction of cytochrome-c and for the effect of temperature on the levels of complex II/III activity from the mitochondria of rat liver. This in-vivo simulation of the reduction of cytochrome-c can be observed using a fibre optic probe which requires less than twenty μl of sample for analysis. The key features of the, system are; front end adaptability, high sensitivity and fast multispectral acquisition which are essential for the biological reactions which are observed.

Original language	English
Pages (from-to)	258-265
Number of pages	8
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	3253
DOIs	https://doi.org/10.1117/12.308038
Publication status	Published - 1998
Externally published	Yes
Event	Biomedical Sensing and Imaging Technologies - San Jose, CA, United States Duration: 26 Jan 1998 → 27 Jan 1998

Keywords

Cytochrome-c
Dip-probe
Fibre optics
Microspectrophotometry
Mitochondrial myopathies
Redox electron carriers
Temperature control

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10.1117/12.308038

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title = "Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes",

abstract = "We report the development of a microspectrophotometer system for use on micro samples of mitochondrial respiratory pigments. A novel optical fibre set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Data is presented for the reduction of cytochrome-c and for the effect of temperature on the levels of complex II/III activity from the mitochondria of rat liver. This in-vivo simulation of the reduction of cytochrome-c can be observed using a fibre optic probe which requires less than twenty μl of sample for analysis. The key features of the, system are; front end adaptability, high sensitivity and fast multispectral acquisition which are essential for the biological reactions which are observed.",

keywords = "Cytochrome-c, Dip-probe, Fibre optics, Microspectrophotometry, Mitochondrial myopathies, Redox electron carriers, Temperature control",

author = "Kavanagh, \{K. Y.\} and Walsh, \{J. E.\} and J. Murphy and M. Harmey and Farrell, \{M. A.\} and O. Hardimann and R. Perryman",

year = "1998",

doi = "10.1117/12.308038",

language = "English",

volume = "3253",

pages = "258--265",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

note = "Biomedical Sensing and Imaging Technologies ; Conference date: 26-01-1998 Through 27-01-1998",

}

TY - JOUR

T1 - Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes

AU - Kavanagh, K. Y.

AU - Walsh, J. E.

AU - Murphy, J.

AU - Harmey, M.

AU - Farrell, M. A.

AU - Hardimann, O.

AU - Perryman, R.

PY - 1998

Y1 - 1998

N2 - We report the development of a microspectrophotometer system for use on micro samples of mitochondrial respiratory pigments. A novel optical fibre set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Data is presented for the reduction of cytochrome-c and for the effect of temperature on the levels of complex II/III activity from the mitochondria of rat liver. This in-vivo simulation of the reduction of cytochrome-c can be observed using a fibre optic probe which requires less than twenty μl of sample for analysis. The key features of the, system are; front end adaptability, high sensitivity and fast multispectral acquisition which are essential for the biological reactions which are observed.

AB - We report the development of a microspectrophotometer system for use on micro samples of mitochondrial respiratory pigments. A novel optical fibre set-up uses visible spectrophotometry to monitor the reduction of mitochondrial electron carriers. Data is presented for the reduction of cytochrome-c and for the effect of temperature on the levels of complex II/III activity from the mitochondria of rat liver. This in-vivo simulation of the reduction of cytochrome-c can be observed using a fibre optic probe which requires less than twenty μl of sample for analysis. The key features of the, system are; front end adaptability, high sensitivity and fast multispectral acquisition which are essential for the biological reactions which are observed.

KW - Cytochrome-c

KW - Dip-probe

KW - Fibre optics

KW - Microspectrophotometry

KW - Mitochondrial myopathies

KW - Redox electron carriers

KW - Temperature control

UR - https://www.scopus.com/pages/publications/0032223868

U2 - 10.1117/12.308038

DO - 10.1117/12.308038

M3 - Conference article

AN - SCOPUS:0032223868

SN - 0277-786X

VL - 3253

SP - 258

EP - 265

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Biomedical Sensing and Imaging Technologies

Y2 - 26 January 1998 through 27 January 1998

ER -

Temperature control of a microspectrophotometer system for monitoring the redox reactions of respiratory pigments in small volumes

Abstract

Keywords

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