Tentative Identification of Etazene (Etodesnitazene) Metabolites in Rat Serum and Urine by Gas Chromatography-Mass Spectrometry and Accurate Mass Liquid Chromatography-Mass Spectrometry

Andrej Grigoryev, Pierce Kavanagh, Geraldine Dowling, Igor Rodin

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

2-Benzylbenzimidazole derivatives comprise a small but forensically significant group of synthetic opioids. In humans, the metabolism of some members of this group is extensive, with little or none of the parent compound remaining. The recent detection of the 2-benzylbenzimidazole derivative, etazene (etodesnitazene), in products seized in Russia required the detection of its metabolites in biofluids for forensic toxicology purposes. Using gas chromatography--mass spectrometry (GC-MS) and high resolution accurate mass (HRAM) liquid chromatography-mass spectrometry (LC-MS), eight etazene metabolites were found in the urine and serum of rats. These were tentatively identified as products of N-deethylation, O-deethylation, hydroxylation or N-oxidation of benzimidazole moiety and combinations of these processes. The parent substance and its O-deethylated metabolite prevailed in rat serum, while in urine, the level of etazene was low compared to N,O-deethylated and N-deethylated with hydroxylation metabolites. Glucuronidated, sulfonated and glutathionated forms were not found. Taking into account reports on the study of the metabolism of other 2-benzylbenzimidazole derivatives in humans, it may be concluded that the mono-deethylated and mono-hydroxylated metabolites are suitable as target analytes in urine.

Original languageEnglish
Pages (from-to)1032-1037
Number of pages6
JournalJournal of Analytical Toxicology
Volume46
Issue number9
DOIs
Publication statusPublished - 24 Jan 2023

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