Testing and validating a modified CTAB DNA extraction method to enable molecular parentage analysis of fertilized eggs and larvae of an emerging South African aquaculture species, the dusky kob Argyrosomus japonicus

L. Mirimin; R. Roodt-Wilding

doi:10.1111/jfb.12639

Testing and validating a modified CTAB DNA extraction method to enable molecular parentage analysis of fertilized eggs and larvae of an emerging South African aquaculture species, the dusky kob Argyrosomus japonicus

L. Mirimin
, R. Roodt-Wilding

Department of Natural Resources & The Environment

Stellenbosch University

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

This study describes the successful implementation of a modified cetyltrimethyl ammonium bromide (CTAB) protocol to isolate genomic DNA and amplify 14 microsatellite markers from fertilized eggs and larvae of an emerging South African farmed marine fish species, the dusky kob Argyrosomus japonicus. To test and validate the efficiency of this method, genetic data were utilized to resolve parentage and kinship of first-generation (F1) offspring produced in mass-spawning events of wild broodstock fish in a commercial hatchery.

Original language	English
Pages (from-to)	1218-1223
Number of pages	6
Journal	Journal of Fish Biology
Volume	86
Issue number	3
DOIs	https://doi.org/10.1111/jfb.12639
Publication status	Published - 1 Mar 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Keywords

F1
First generation
Genomic DNA isolation
Kabeljou
Microsatellite loci
Pedigree analyses

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title = "Testing and validating a modified CTAB DNA extraction method to enable molecular parentage analysis of fertilized eggs and larvae of an emerging South African aquaculture species, the dusky kob Argyrosomus japonicus",

abstract = "This study describes the successful implementation of a modified cetyltrimethyl ammonium bromide (CTAB) protocol to isolate genomic DNA and amplify 14 microsatellite markers from fertilized eggs and larvae of an emerging South African farmed marine fish species, the dusky kob Argyrosomus japonicus. To test and validate the efficiency of this method, genetic data were utilized to resolve parentage and kinship of first-generation (F1) offspring produced in mass-spawning events of wild broodstock fish in a commercial hatchery.",

keywords = "F1, First generation, Genomic DNA isolation, Kabeljou, Microsatellite loci, Pedigree analyses",

author = "L. Mirimin and R. Roodt-Wilding",

note = "Publisher Copyright: {\textcopyright} 2015 The Fisheries Society of the British Isles.",

year = "2015",

month = mar,

day = "1",

doi = "10.1111/jfb.12639",

language = "English",

volume = "86",

pages = "1218--1223",

journal = "Journal of Fish Biology",

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Testing and validating a modified CTAB DNA extraction method to enable molecular parentage analysis of fertilized eggs and larvae of an emerging South African aquaculture species, the dusky kob Argyrosomus japonicus. / Mirimin, L.; Roodt-Wilding, R.
In: Journal of Fish Biology, Vol. 86, No. 3, 01.03.2015, p. 1218-1223.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Testing and validating a modified CTAB DNA extraction method to enable molecular parentage analysis of fertilized eggs and larvae of an emerging South African aquaculture species, the dusky kob Argyrosomus japonicus

AU - Mirimin, L.

AU - Roodt-Wilding, R.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - This study describes the successful implementation of a modified cetyltrimethyl ammonium bromide (CTAB) protocol to isolate genomic DNA and amplify 14 microsatellite markers from fertilized eggs and larvae of an emerging South African farmed marine fish species, the dusky kob Argyrosomus japonicus. To test and validate the efficiency of this method, genetic data were utilized to resolve parentage and kinship of first-generation (F1) offspring produced in mass-spawning events of wild broodstock fish in a commercial hatchery.

AB - This study describes the successful implementation of a modified cetyltrimethyl ammonium bromide (CTAB) protocol to isolate genomic DNA and amplify 14 microsatellite markers from fertilized eggs and larvae of an emerging South African farmed marine fish species, the dusky kob Argyrosomus japonicus. To test and validate the efficiency of this method, genetic data were utilized to resolve parentage and kinship of first-generation (F1) offspring produced in mass-spawning events of wild broodstock fish in a commercial hatchery.

KW - F1

KW - First generation

KW - Genomic DNA isolation

KW - Kabeljou

KW - Microsatellite loci

KW - Pedigree analyses

UR - https://www.scopus.com/pages/publications/84924853590

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DO - 10.1111/jfb.12639

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SN - 0022-1112

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SP - 1218

EP - 1223

JO - Journal of Fish Biology

JF - Journal of Fish Biology

IS - 3

ER -

Testing and validating a modified CTAB DNA extraction method to enable molecular parentage analysis of fertilized eggs and larvae of an emerging South African aquaculture species, the dusky kob Argyrosomus japonicus

Abstract

UN SDGs

Keywords

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