The characterisation of synthetic and natural-product pharmaceuticals by electrospray ionisation-mass spectrometry (ESI-MS) and liquid chromatography (LC)-ESI-MS

W. F. Smyth, S. McClean, C. J. Hack, V. N. Ramachandran, B. Doherty, C. Joyce, F. O'Donnell, T. J. Smyth, E. O'Kane, P. Brooks

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

This article considers the application of topical analytical techniques - electrospray ionisation-mass spectrometry (ESI-MS) and liquid chromatography (LC)-ESI-MS - to the characterisation of selected small molecular-mass synthetic and natural-product pharmaceuticals, as well as bioactive peptides. We chose the synthetic drugs according to selected structural classes in which they give ESI signals primarily as [M + H]+ ions. The structural classes chosen to illustrate the application of ESI-MS and LC-ESI-MS to such drug characterisation are drugs with amine-containing side chains, drugs with N-containing saturated ring structures, and 1,4-benzodiazepines and other heterocyclic hypnotics. We then discuss potential natural-product pharmaceuticals, such as selected quinolines and nicotines. We next apply the technique to the characterisation of molecules of unknown structure present in plant extracts or fractions that possess antibacterial activity. Finally, we discuss the isolation and the characterisation of bioactive peptides from frog-skin secretions utilising an arsenal of MS tools as well as Edman degradation sequencing and cDNA cloning.

Original languageEnglish
Pages (from-to)572-582
Number of pages11
JournalTrAC - Trends in Analytical Chemistry
Volume25
Issue number6
DOIs
Publication statusPublished - Jun 2006
Externally publishedYes

Keywords

  • Bioactive peptide
  • Drug characterisation by LC-ESI-MS
  • Drugs of small molecular mass
  • ESI-MS
  • Electrospray ionisation-mass spectrometry
  • Fragmentation pattern
  • LC-ESI-MS
  • Liquid chromatography-electrospray ionisation-mass spectrometry
  • Natural-product pharmaceutical

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