The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes

Lisong Yang; Cian M. Taylor; Yury Rakovich; Eithne M. McCabe

doi:10.1117/12.477978

The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes

Lisong Yang
, Cian M. Taylor
, Yury Rakovich
, Eithne M. McCabe

Trinity College Dublin

Research output: Contribution to journal › Conference article › peer-review

Abstract

Images of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.

Original language	English
Pages (from-to)	66-72
Number of pages	7
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4964
DOIs	https://doi.org/10.1117/12.477978
Publication status	Published - 2003
Externally published	Yes
Event	Three-Dimensional and Multidimensional Microscopy:Image Acquisition and Processing X - San Jose, CA, United States Duration: 28 Jan 2003 → 29 Jan 2003

Keywords

microsphere
polarization
three-dimensional microscopy

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10.1117/12.477978

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title = "The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes",

abstract = "Images of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.",

keywords = "microsphere, polarization, three-dimensional microscopy",

author = "Lisong Yang and Taylor, \{Cian M.\} and Yury Rakovich and McCabe, \{Eithne M.\}",

year = "2003",

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journal = "Proceedings of SPIE - The International Society for Optical Engineering",

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T1 - The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes

AU - Yang, Lisong

AU - Taylor, Cian M.

AU - Rakovich, Yury

AU - McCabe, Eithne M.

PY - 2003

Y1 - 2003

N2 - Images of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.

AB - Images of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.

KW - microsphere

KW - polarization

KW - three-dimensional microscopy

UR - https://www.scopus.com/pages/publications/0141717147

U2 - 10.1117/12.477978

DO - 10.1117/12.477978

M3 - Conference article

AN - SCOPUS:0141717147

SN - 0277-786X

VL - 4964

SP - 66

EP - 72

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Three-Dimensional and Multidimensional Microscopy:Image Acquisition and Processing X

Y2 - 28 January 2003 through 29 January 2003

ER -

The three-dimensional imaging of microspheres in confocal and conventional polarization microscopes

Abstract

Keywords

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