Three-dimensional imaging of microspheres with confocal and conventional polarization microscopes

Cian Taylor; Lisong Yang; Yury Rakovich; Eithne M. McCabe

doi:10.1364/ao.42.005693

Three-dimensional imaging of microspheres with confocal and conventional polarization microscopes

Cian Taylor
, Lisong Yang
, Yury Rakovich
, Eithne M. McCabe

Trinity College Dublin

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

mages of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.

Original language	English
Journal	Applied Optics
DOIs	https://doi.org/10.1364/ao.42.005693
Publication status	Published - 9 Jul 2003
Externally published	Yes
Event	SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X: Biomedical Optics - San Jose, United States Duration: 9 Jul 2023 → 9 Jul 2023

Keywords

birefringence
light polarization
optical microscopy
scanning
signal detection

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10.1364/ao.42.005693

Cite this

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title = "Three-dimensional imaging of microspheres with confocal and conventional polarization microscopes",

abstract = "mages of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.",

keywords = "birefringence, light polarization, optical microscopy, scanning, signal detection",

author = "Cian Taylor and Lisong Yang and Yury Rakovich and McCabe, \{Eithne M.\}",

year = "2003",

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language = "English",

journal = "Applied Optics",

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TY - JOUR

T1 - Three-dimensional imaging of microspheres with confocal and conventional polarization microscopes

AU - Taylor, Cian

AU - Yang, Lisong

AU - Rakovich, Yury

AU - McCabe, Eithne M.

PY - 2003/7/9

Y1 - 2003/7/9

N2 - mages of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.

AB - mages of the microspheres are studied in three-dimensions by using the confocal and conventional scanning polarization microscopes. It is found that the polarization of the detected signals is mainly parallel to the initial polarization which is due to the high extinction coefficient of the confocal system. Arc pairs are observed at the edge of the microspheres with the conventional polarization microscope with a crossed analyzer. Theoretical analysis are given by using the vector field theory and the image formations of the two systems.

KW - birefringence

KW - light polarization

KW - optical microscopy

KW - scanning

KW - signal detection

U2 - 10.1364/ao.42.005693

DO - 10.1364/ao.42.005693

M3 - Article

SN - 1559-128X

JO - Applied Optics

JF - Applied Optics

T2 - SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X

Y2 - 9 July 2023 through 9 July 2023

ER -

Three-dimensional imaging of microspheres with confocal and conventional polarization microscopes

Abstract

Keywords

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